Basic Information
Poster Number: 1780-9    
Author Name: Thomas J Waeghe Affiliation: MAC MOD Analytical, Inc.
Session Title: Liquid Chromatography Applications
Event Type: Poster
Event Title: Bridging the Gap Between UHPLC and HPLC: Easy Method Transfer Using Fused-Core Columns
Presider(s):   Start Time: ( Slot # 9 )
Date: Wednesday, March 14th, 2012 Location: Blue Area on Exposition Floor, Aisles 3300-3500
Keywords: HPLC, HPLC Columns

Schuster, Stephanie AAdvanced Materials Technology

Abstract Content
Many chromatographers are now exploiting the speed and efficiency advantages of UHPLC as part of their method development scheme. The use of low dispersion UHPLC instrumentation and highly efficient columns allows them to screen different column stationary phases and analysis conditions (pH, organic modifier, temperature, etc.) much more quickly than ever before. Moreover, with this approach the resulting high speed and/or high resolution method will be more robust and efficient, and will be able to generate analytical results that enable faster and better decisions with high productivity.

However, when it is necessary to transfer such methods to a quality control or production environment, method developers often must cope with the limited availability of UHPLC instrumentation and operator expertise in such laboratories. Often, it is necessary to transfer the method to a longer and larger ID column with a much larger particle size to be able to transfer the method to conventional instrumentation (300–400 bar pressure limit).

UHPLC columns with 2.7 m particle size can deliver performance comparable to sub-2-m columns at 40–50% of the back pressure. This benefit makes method transfer much easier between UHPLC and HPLC systems. It is only necessary to make modifications to the extracolumn volume and dispersion of the HPLC system, with some additional adjustments to the analysis conditions.

We will discuss the parameters that must be considered and adjusted when transferring isocratic and gradient methods from UHPLC instruments to conventional HPLC systems. Isocratic and gradient examples will be shown in which Fused-Core
UHPLC separations are transferred to various HPLC instruments having different extracolumn dispersion and different delay volumes. Guidance will be offered to make such method transfers more successful.