Basic Information
Abstract Number: 890-4    
Author Name: David C Muddiman Affiliation: North Carolina State University
Session Title: Pittsburgh Analytical Chemistry Award - WEBCASTING
Event Type: Award
Event Title: Rational Biomarker Discovery and Validation Using a Systems Approach
Presider(s): Jackovitz, John Start Time: 10:10 AM ( Slot # 7 )
Date: Tuesday, March 13th, 2012 Location: 300
Keywords: Biomedical, Carbohydrates, Mass Spectrometry, Proteomics

Abstract Content
Mass-spectrometry has the ability to elucidate new diagnostic, prognostic, and therapeutic biomarkers and translate them to the clinic. However, there are numerous challenges that exist that must be overcome. This presentation will discuss the challenges specific to the study of epithelial ovarian cancer (EOC) in humans and how these challenges have directed our thinking in terms of the development of mass spectrometry-based bioanalytical strategies. First, to augment the human model, we have developed a chicken model of spontaneous EOC which allows us to control the environment and genetic background, a model which is characterized by rapid onset and progression of disease, and allows us to conduct longitudinal sampling. Second, we have developed hydrophobic tagging reagents to increase the electrospray response of N-linked glycans with a major benefit of being able to incorporate a stable-isotope label for use in relative quantification experiments. Third, we have utilized targeted metabolomic data to augment the proteome and glycome data. Finally, we have developed methods for whole tissue and laser-capture micro dissection of proteome analysis of tissue, and new ionization methods that can be used for direct analysis and tissue imaging; the latter being a fruitful arena for biomarker discovery.