Basic Information
Abstract Number: 1940-1    
Author Name: Jessica S Creamer Affiliation: University of Kansas
Session Title: Detection Strategies for Microfluidic Devices
Event Type: Organized Contributed Session
Event Title: On-Chip Analysis of Pharmaceuticals Using Capacitively Coupled Contactless Conductivity Detection
Presider(s): da Silva, Jose Alberto Fracassi Start Time: 02:00 PM ( Slot # 1 )
Date: Wednesday, March 14th, 2012 Location: 207B
Keywords: Detection, Electrochemistry, Electrophoresis, Pharmaceutical

da Silva, Jose Alberto FUniversidade Estadual de Campinas
Lunte, Susan MUniversity of Kansas

Abstract Content
Assuring quality control of pharmaceuticals in developing countries is an important challenge in global health today. Unfortunately, neither funding nor infrastructure is available to support centralized lab facilities in locations where testing is most needed. The overall goal of this project is to investigate microchip electrophoresis (ME) as a screening method for counterfeit drugs in developing countries. Microchips are ideal for use in such locations because they are small, portable, and disposable. In ME, the small channel dimensions provide fast analysis times as well as reducing reagent consumption and the waste that is generated. By fabricating the device from inexpensive plastics and polymers the overall cost-per-test can be made more affordable than traditional lab equipment. Additionally, by coupling the microchip to a universal detection system the device could be applied towards quality control of many pharmaceutical without having to change the technology platform.

This project concerns the evaluation of ME coupled with capacitively coupled contactless conductivity detection (C4D) for the separation and detection of pharmaceuticals commonly distributed in developing countries. C4D is a universal detector that measures the change in conductivity of the solution in the separation channel as charged analyte bands move past the electrodes.

Initial results include a separation of the first-line anti-tuberculosis drug ethambutol from its major degradation product (2-amino-1-butanol) using a PDMS/glass 5cm simple-t microchip. On-going work is focused on developing a ME separation and C4D detection for all four first-line anti-tuberculosis drugs. In the near future we hope to be able to use this microchip and detection system to study a range of pharmaceuticals from NSAIDs to biopharmaceuticals.

We acknowledge the Biotech Training Grant (T32-GM008359) NIGMS; NINDS R01 42929; and the Adams Institute for Bioanalytical Chemistry for financial support.